Haemacytometer



Jan. 8, 1924. 1,480391 C. A. HA'USSER HEMACYTOMETER Filed April 11. 1921 Patented J an. 8, 1924.

UNITED STATES 1,480,391; PATENT OFFICE.

CARL ADOLPH' EAUSSER, OF PHILADELPHIA, PENN SYLVANIA, ASSIGNOR '.l'.'0 C. r A.

HAUSSER & SON, OF PHILADELPHIA, PENNSYLVANIA, A FIRE GONSIS'IING OF CARL APOLCPH HAUSSEB LAND ANTHONY ADOLPH HAUSSER.

HEMACYTOMETER.

Application filed April 11, 1921. Serial No. 480,564.

To all whom it may concern.-

Be it known that I, CARL ADOLPH I- IA Ussnn, a citizen of the United States, residing in Philadelphia Pennsylvania, have ina vented certain mprovements in Hmmacytometers, of which the following is a speclfication.

My invention relates to improvements in hsemacytometers, one object of the invention 1 being to provide a supporting and protective frame or casing, said frame preferably being such that the structure, usually of las's, which constitutes the counting cham-' r, is easily removable therefrom and reinsertable therein, this construction having further in view the provision in connection with said frames of sets of counting chamber structures, including all forms suited to the various uses of the device, all of the 90 structures of the set being adapted to the said frame andinterchangeable therein.

Another object of the invention is to provide novel and improved means for supporting the cover plate above the counting chamber, insuring an accurate disposition of the said cover plate relative to the countmg chamber.

A. still furtherobject is to provide means for clamping the cover plate in position over the counting chamber, said clamping means being such as to insure the retention of the cover plate in place with no distortion of the cover plate.

The manner in which these and other objects appearing hereinafter are attained is illustrated in the attached drawings, in) which:

Figure 1 is a view in perspective of an assembled semacytometer made in accord- 40 ance. with my invention;

Fig 2, is a view in perspective of a single counting chamber structure de-' tached from the frame;

Fig. 3, is a side elevation of my improved haemacytometer;

Fig. 4:, is a sectional elevation taken on the line 44, Fig. 3;

Fig. 5, is an inverted perspective of the frame, and

Fig. 6 is a view in'perspective of double counting chamber structure.

With reference to the drawings, Fig. 1.

illustrates a preferred form of my protective frame, said frame in the present 1nand which thereby is adapted to receive a stance consisting of a body portion 1 hav- 56 mg in the face thereof and extending from one end to a point short of the other end, a

longitudinal recess 2, the opposite longitudmal-sides' 3, 3, of which are-beveled,

60 counting chamber structure 4 of the type shown 1n Fig. 2, whose side edges 5, 5, are beveledto correspond with the beveled edges of the said recess, said structure 4 being adapted to be slid into the recess from the end of the frame. Also adapted to. occupy the said recess is a follower 6' havin its edges beveled in the manner of .those 0 the counting chamber structure and being adapted also to be slid into place in the outer end of the said recess after insertionof the counting chamber structure, thereby to retain thesaid structure firmly in the inner end of the recess: A suitable retainingdevice is provided for locking the said f0 lower 6 in place in the recess'and to prevent .outward movement thereof, said retainingielement in the present instance con- ,sisting iof a latch member 7 pivoted at one end to one side of the end surface of said frame and having a book 8 at the outer or free end which is adapted to en age a suit pedestals 11 which are trued up in such fashionthat when the frame rests on a plane surface the face of the counting chamber is disposed parallel with the said supporting surface.

It will be clear that with a device of this nature not only is superior protection afforded to thecomparatively fragile counting chamber structure, but the assembled device may be handlechwith great-ease. 1 have found that bakelite is an excellent material of which to form the rotecting frame of the haemacytometer, 'alt ough any material suitable for the purpose may be employed.

It will be noted that the counting chamber structures are of the same general form.

as those shown and described in the patent to Max Levy No. 1,214,331, dated January 30, 1917, with the exception that the body of the structure is reduced to the minimum dimensions required by the counting chamber, this reduction in size, of course, being made possible by the use of the rotective frame previously described.

As clearly illustrated, the counting chamber structure consists of a body portion 12 having at a side thereof spaced parallel channels .13, 13, extend-ing completely across the body, that portion 14 of the body lying between the said channels having its sur-- face slightl. lower than the top surfaces of the two si e portions 15, 15, which bound the outer sides of the said channels. The

1 two side portions are comparatively narrow, and as shown in Fig. 6, I'may, where a double counting chamber is desired, provide a cross channel 16 substantially the depth of the said channels 13 and extending through the side portions. 15 and intermediate .portion 14 and being preferably centrally disposed and at right angles to the said'channels v13. This cross channel 16 I make of generouswidth, and so am, able ,to prevent the passage of a liquid from one side of the counting chamber to the other side, as often happens in the double counting chambers of the usual form due to capillary attraction, since any excess blood or fluid entering the said channels 13 and 16 may pass ofl from the counting chamber through the side portions 15 byreason of the said channel 16 which, as previously described, extends. therethrough. This has been found a decided advantage.

I also provide means for insuring the accurate support of the cover plate 20 above the counting chamber, this means consistmgi of a pluralit of elevated supports or pe estals 21 on t e side portions 15, upon which-pedestals or supports the said cover plate is. adapted to rest. Thus, for example, in the double cdunting chamber illustrated in Fig. 6, I prcrvide four pedestals, two oneach of the side portions and disposed directly opposite the rulings 22 on the central part of the chamber which define the area in which the counting is tobe done. In the case of the singlechamber shown in Fig. 2, I prefer to empl oya three 'point support, one of the supporting pedestals 21 bein established on one of the side. portions i '15 directly in line with the ruling 22 on the central portion of the chamber, and the "other two pedestals being evenly spaced apart upon the opposite side portion of the counting chamber.

Means is also provided for clamping the cover plate in position above thecountin chamber, said means being in the form 0 1'esilientclips 25 of generall vU-shape formation which are adapted to slippedbvcr said clips being two in number and being adapted to be placed on opposite sides of the frame, preferably at thepoints of the pedestals 21, as most clearly shown in Fig. 1. This form of clamp in no way interferes with the ;-manipulation of the haemacytometer, and while clamping. the cover plate firmly in position, cannot distort the cover plate in. any manner, particularly when properly applied so as to overlie the said supporting pedestals.

I claim:

1. In a haemacytometcr, a slide comprising a frame of relatively non-breakable ma terial, and a counting chamber structure detachably secured. in said frame.

2. In a heemacytometer, a slide com rising a frame of non-fragile materia a counting chamber structure mounted in the "frame, and positive locking means on the frame insuring retention of the counting chamber.

3. A haemacytometer consisting of a slide including a counting chamber, that portion. of the slide constituting the counting cham her being detachable; substantially as de scribed. 1.

4. Ina heemacytometer, a slide compris countipg' chamber adapted to be detachably retain in the frame over said aperture, and positive locking means on the frame insuring retention of the counting-chamber,

6. In a haemacytometer, the combination" with a structure constituting a counting chamber, of a frame having a central 8.1161". ture therein, and a recess in one slde extend- 'ing' inwardly from an edge of the frame,

' of 'a translucent structure constituting .a

thesaid counting chamber structure and recess having intereng'aging side edges adapted to retain the-said structure in the recess after insertion, a follower insertable in said recess for retaining the said structure inp'osition overlyin said aperture, and means for reta ning t c said follower in the recess.-

7. In a heemacyt'ometer, the combination with a structure constituting a countin chamber, of a frame adapted .to'receive an detachably retain said structure, and having at the 'bottom-.ther'eof a plurality of spaced promotions adapted to elevate the body of the frame above agsupporting surface.

8. A heemacytometer comprising. a. body having in'the face'thereof two spaced parallel channels bounding an area constituting the counting chamber or chambers, and a channel intersecting said parallel channels and dividing the said chamber area and extending from one edge of the body to the other.

9. A heemacytometer comprising a counting chamber structure having three substantiallyparallel. portions separated by a pair of spaced channels, and elevations upon the outer of said parallel portions constituting a support for a cover'plate over the counting chamber. I q 10. Aheemacytometer comprising acounting chamber structure having three substantially parallel portions separated by a pair of Spaced channels, a plurality of elevations upon the outer of said parallel portions, a cover plate resting upon said elevations, and independent means acting at the .ing translucent, and the remainder of the 2 slide being opaque.

CARL ADOLPH HAUSSER. I

points of saidelevations for clamping the 20- All Ac 

